WebA gene encoding a putative guanosine 5′-diphosphate (or 5′-triphosphate) 3′-diphosphate ((p)ppGpp) synthetase, designated PepRSH (Pepper RelA/SpoT homologue), was isolated from hot peppers. PepRSH was found to contain five introns and six exons and a 2166-bp open reading frame encoding a protein of 721 amino acids; this protein displayed … WebApr 14, 2024 · Protein-biomolecule interactions play pivotal roles in almost all biological processes. For a biomolecule of interest, the identification of the interacting protein(s) is essential. For this need, although many assays are available, highly robust and reliable methods are always desired. By combining a substrate-based proximity labeling activity …
Recent functional insights into the role of (p)ppGpp in bacterial ...
WebJan 15, 2024 · The (p)ppGpp synthetase domain catalyzes the production of ppGpp and pppGpp through the transfer of pyrophosphate from ATP to the ribose 3′-OH of GDP or … WebOct 12, 2015 · To determine the allosteric effects of ppGpp and pppGpp on SAS1 (p)ppGpp synthetase activity, 2 µM of SAS1 were incubated in a total volume of 50 µL in a reaction … mohave county employee
Evaluation of the probiotic attributes of Bacillus strains isolated ...
WebAlthough several chloroplast RSHs are widely distributed in plants 119, the only other eukaryotes that have so far been found to encode cytoplasmic (p)ppGpp synthetase … WebJul 19, 2024 · Several genes involved in respiration, including those to the FARAD o F 1 ATP synthase, ... (p)ppGpp. Although that tight response has not been studied in P. mirabilis, the genome for wild-type BB2000 comprises two canonical genes for production and degradation of ppGpp, relA both spoT . WebThese various levels of regulation involve the participation of signaling molecules such as the γ-butyrolactones and (p)ppGpp, activator and repressor proteins, two-component regulatory systems, RNA polymerase sigma factors and anti-sigma factors, regulatory RNAs and other regulatory molecules (Bibb, 1996; Liu et al., 2013; Niu et al., 2016). mohave county election ballot