WebThese primers can also be modified to target different regions of the 16S gene, or altered for custom applications. The 27 samples from the reservoir were prepared using the 16S library preparation protocol and the Nextera® XT DNA Index Kit6 for cost-effective sample multiplexing. Sequencing The MiSeq System can deliver 2 × 300 bp reads and up to
Nextera XTDNA SamplePreparationGuide - University of Hawaiʻi
WebNov 29, 2010 · Nextera kits were designed to prepare libraries from genomic DNA; however, with minor modifications, the technology can be adapted for RNA-seq (cDNA) library preparation. A random-primed method... WebSequencing Nextera XT libraries on the HiSeq 1000/2000, HiSeq 1500/2500 in High Output mode, GA, or HiScanSQ requires one of the following sequencing primer boxes. These primers are included in the SBS reagents for the MiniSeq, MiSeq, NextSeq, HiSeq Rapid Run mode, and HiSeq 3000/HiSeq 4000. east ayrshire council young scot card
Illumina 16S Metagenomic Sequencing Protocol
WebWith Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single-tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It supports a wide range of … Webprimer dimers. Run 1 µl of library on a Bioanalyzer using a high-sensitivity DNA chip to check library size. Alternatively, PCR amplify the library using adapter primers and run on an agarose gel to visualize the presence of library. Adapter primer sequences can be found on page 6 of the Sequencing Library Quantification Guide (part# 11322363). WebNote: The kit contains a 50X Nextera Primer Cocktail, which consists of Primer 1 (10 μM), Primer 2 (10 μM), and Adaptor 1 (0.5 μM). A single primer, 50X Nextera Adaptor 2 … east ayrshire cpd service